1Q5P
S156E/S166D variant of Bacillus lentus subtilisin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.450, 61.500, 75.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 24874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 2.0 (mg/ml) | |
| 2 | 1 | 1 | propylene glycol | 50 (%) |
