1Q5O
HCN2J 443-645 in the presence of cAMP, selenomethionine derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96429 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 95.429, 95.429, 50.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.216 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q43 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.190 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.100 | 2.300 |
| Rmerge | 0.069 | |
| Number of reflections | 13749 | |
| <I/σ(I)> | 23.62 | |
| Completeness [%] | 98.0 | 99.5 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | PEG 400, sodium citrate, sodium chloride, DTT, HEPES, cAMP, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 200 (mM) | ||
| 2 | 1 | reservoir | citrate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | PEG400 | 15 (%) | |
| 4 | 1 | drop | protein | 2.3 (mg/ml) |
