1Q5A
S-shaped trans interactions of cadherins model based on fitting C-cadherin (1L3W) to 3D map of desmosomes obtained by electron tomography
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Staining material | Osmium tetroxide and Uranyl Acetate |
EM Data Collection
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GENERIC GATAN |
3D Reconstruction
| Method | TOMOGRAPHY |
| Resolution (Å) | 30 |
| Software | IMOD,Amira |
| Symmetry | C1 |
