1Q56
NMR structure of the B0 isoform of the agrin G3 domain in its Ca2+ bound state
Spectrometer
Experimental method: SOLUTION NMR
Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
1 | Varian | INOVA | 600 |
Experiment
experiment id | conditions id | solution id | Experiment type |
1 | 1 | 1 | 3D_15N-separated_NOESY (100 ms) |
2 | 1 | 1 | HNHB (chi restraints) |
3 | 1 | 1 | HSQC-NOESY-HSQC (tm = 200 ms / perdeuterated protein) |
4 | 1 | 1 | HNCACB (TALOS restraints from HN,Ca, Cb shifts ), HNCO (Talos C' shifts), 3D-15N sep TOCSY (Talos Ha shifts) |
5 | 1 | 2 | 3D-13C separated NOESY (tm = 125 ms) and isotope exchange experiments to derive H-bond restraints |
NMR Sample
conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
1 | 6.5 | ambient | 298 |
Conformers
Conformers Calculated Total Number | 400 |
Conformers Submitted Total Number | 15 |