1Q4V
CRYSTAL STRUCTURE OF ALLO-ILEA2-INSULIN, AN INACTIVE CHIRAL ANALOGUE: IMPLICATIONS FOR THE MECHANISM OF RECEPTOR
Replaces: 1PC1Replaces: 1LW8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.487, 80.487, 37.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.200 * - 2.000 |
| Rwork | 0.219 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.200 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.301 |
| Number of reflections | 5892 * | 543 * |
| <I/σ(I)> | 43.1 | 4.1 |
| Completeness [%] | 98.7 | 89.3 |
| Redundancy | 2.07 | 1.27 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 290 | Tris, sodium citrate, acetone, phenol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 0.02 (M) | ||
| 3 | 1 | reservoir | Tris-HCl | 0.02 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.05 (M) | |
| 5 | 1 | reservoir | acetone | 5 (%) | |
| 6 | 1 | reservoir | phenol | 0.03 (%) | |
| 7 | 1 | reservoir | zinc acetate | 0.01 (%) | pH8.0 |
