1Q1T

Mouse Importin alpha: non-phosphorylated SV40 CN peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-13
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	79.157, 89.282, 100.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500
R-factor	0.202
Rwork	0.202
R-free	0.23300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ial
RMSD bond length	0.007 *
RMSD bond angle	19.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.078	0.255
Total number of observations	443936 *
Number of reflections	25902
<I/σ(I)>	19.8	3.9
Completeness [%]	97.2	88.6
Redundancy	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.25	293	Sodium Citrate, DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	1.7 (mg/ml)
2	1	drop	sodium citrate	0.7-0.8 (M)
3	1	reservoir	HEPES	100 (mM)	pH6.0-6.5
4	1	reservoir	dithiothreitol	10 (mM)