1Q1T
Mouse Importin alpha: non-phosphorylated SV40 CN peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.157, 89.282, 100.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ial |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 19.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.255 |
| Total number of observations | 443936 * | |
| Number of reflections | 25902 | |
| <I/σ(I)> | 19.8 | 3.9 |
| Completeness [%] | 97.2 | 88.6 |
| Redundancy | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.25 | 293 | Sodium Citrate, DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 1.7 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 0.7-0.8 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH6.0-6.5 |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
