1PZ8
Modulation of agrin function by alternative splicing and Ca2+ binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7B |
Temperature [K] | 100 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.847, 56.556, 84.586 |
Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.350 |
R-factor | 0.21991 |
Rwork | 0.218 |
R-free | 0.26023 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.535 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.800 |
High resolution limit [Å] | 2.350 |
Number of reflections | 29221 |
<I/σ(I)> | 11.3 |
Completeness [%] | 97.1 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 276 | PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K |