1PXG
Crystal structure of the mutated tRNA-guanine transglycosylase (TGT) D280E complexed with preQ1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.280, 64.940, 70.200 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.1594 |
| Rwork | 0.159 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.027 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.253 |
| Total number of observations | 155496 * | |
| Number of reflections | 42492 | |
| <I/σ(I)> | 24.3 | 6.1 |
| Completeness [%] | 94.5 | 90.6 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 25. * | Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 13 (%) | |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | agarose | 0.2 (%) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 6 | 1 | drop | 2 (M) | ||
| 7 | 1 | drop | dithiothreitol | 1 (mM) | |
| 8 | 1 | drop | protein | 12 (mg/ml) |
