1PX8
Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.9500, 0.9787, 0.9789, 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.719, 92.719, 241.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 * | 0.247 * |
| Total number of observations | 172649 * | |
| Number of reflections | 37528 * | |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 89.1 | 76.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 * | Yang, J.K., (2002) Acta Crystallogr., D58, 531. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | D-xylose | 1.0 (M) | |
| 3 | 1 | reservoir | PEG2000MME | 24 (%(w/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
