1PVY
3,4-dihydroxy-2-butanone 4-phosphate synthase from M. jannaschii in complex with ribulose 5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.197, 69.272, 57.110 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.223 |
| Rwork | 0.213 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pvw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.476 |
| Number of reflections | 42575 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 93.6 | 97.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 293 | Fischer, M., (2002) J. Biol. Chem., 277, 41410. * |
