1PV6
Crystal structure of lactose permease
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.351, 125.845, 188.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.500 |
| R-factor | 0.294 |
| Rwork | 0.294 |
| R-free | 0.33700 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 19.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.075 * | 0.461 * |
| Total number of observations | 63863 * | |
| Number of reflections | 25917 * | |
| Completeness [%] | 85.9 * | 71.8 |
| Redundancy | 2.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 2 | 1 | reservoir | PEG400 | 27-30 (%) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 1,6-hexanediol | 3 (%) | |
| 5 | 1 | reservoir | CHAPS | 0.8 (mM) |
