1PT7
Crystal structure of the apo-form of the yfdW gene product of E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.921, 118.634, 136.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.15985 |
| Rwork | 0.159 |
| R-free | 0.19100 * |
| Structure solution method | SAD |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.470 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.800 * | 1.800 * |
| Rmerge | 0.048 * | 0.268 |
| Number of reflections | 85290 | |
| <I/σ(I)> | 9.8 | 2.8 |
| Completeness [%] | 96.7 * | 96.7 * |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | Ammonium phosphate, sodium hepes, pH 7.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 5 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | ammonium phosphate | 0.9 (M) | |
| 5 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
