1PS3

Golgi alpha-mannosidase II in complex with kifunensine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9504
Spacegroup name	P 21 21 21
Unit cell lengths	69.089, 109.803, 138.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
Rwork	0.200
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.006 *
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.058 *	0.180
Total number of observations	759620 *
Number of reflections	144642
<I/σ(I)>	17.2	4.1
Completeness [%]	94.2 *	83.3
Redundancy	5.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	PEG 6000, MPD, Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	100 (mM)	pH7
2	1	reservoir	PEG6000	8.5 (%)
3	1	reservoir	MPD	2.5 (%)