1PRX
HORF6 A NOVEL HUMAN PEROXIDASE ENZYME
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.850, 75.170, 63.300 |
| Unit cell angles | 90.00, 110.21, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.25900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.790 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.189 |
| Number of reflections | 24439 | |
| <I/σ(I)> | 17 | 6.1 |
| Completeness [%] | 93.7 | 91.4 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 295 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
