1PRO
HIV-1 PROTEASE DIMER COMPLEXED WITH A-98881
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-02-19 |
Detector | RIGAKU |
Spacegroup name | P 61 |
Unit cell lengths | 63.080, 63.080, 83.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.201 * |
Rwork | 0.185 |
R-free | 0.31000 |
RMSD bond length | 0.014 |
RMSD bond angle | 3.100 * |
Data reduction software | R-AXIS ((HIGASHI/RIGAKU)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 * | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.050 | |
Number of reflections | 16426 | |
Completeness [%] | 93.0 | 85 * |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium salfate | 20 (%) | |
2 | 1 | reservoir | sodium acetate | ||
3 | 1 | drop | DMSO | 2 (%) | |
4 | 1 | drop | protease | 2-2.5 (mg/ml) | |
5 | 1 | drop | N-ethylmorpholine | 0.1 (M) |