1PRE
PROAEROLYSIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1989-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.000, 104.000, 222.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.208 * |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.044 |
| Data reduction software | MOSFLM |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.091 |
| Total number of observations | 145443 * |
| Number of reflections | 30060 |
| Completeness [%] | 98.0 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 * | Tucker, A.D., (1990) J.Mol.Biol., 212, 561. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.0 (mg/ml) | |
| 2 | 1 | reservoir | acetate | 100 (mM) | pH5.4 |
| 3 | 1 | reservoir | PEG4000 | 1-2 (%) |
