1PRE
PROAEROLYSIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Detector technology | IMAGE PLATE |
Collection date | 1989-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 104.000, 104.000, 222.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.800 |
R-factor | 0.208 * |
RMSD bond length | 0.013 |
RMSD bond angle | 0.044 |
Data reduction software | MOSFLM |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.091 |
Total number of observations | 145443 * |
Number of reflections | 30060 |
Completeness [%] | 98.0 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.4 * | Tucker, A.D., (1990) J.Mol.Biol., 212, 561. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.0 (mg/ml) | |
2 | 1 | reservoir | acetate | 100 (mM) | pH5.4 |
3 | 1 | reservoir | PEG4000 | 1-2 (%) |