1PQ3
Human Arginase II: Crystal Structure and Physiological Role in Male and Female Sexual Arousal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 |
Unit cell lengths | 142.994, 142.994, 127.328 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.700 | |
Rmerge | 0.098 * | 0.280 * |
Total number of observations | 404391 * | |
Number of reflections | 79653 * | |
Completeness [%] | 99.3 * | 98.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | Tris, Ammonium Sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | arginase | 7 (mg/ml) | |
2 | 1 | drop | BEC | 5 (mM) | |
3 | 1 | drop | Bicine | 50 (mM) | pH8.5 |
4 | 1 | drop | Tris-HCl | 0.10 (M) | pH8.3-8.5 |
5 | 1 | drop | ammonium sulfate | 3.0 (M) | |
6 | 1 | reservoir | Tris-HCl | 0.10 (M) | pH8.3-8.5 |
7 | 1 | reservoir | ammonium sulfate | 3.0 (M) | |
8 | 1 | reservoir | glycerol | 20 (%(v/v)) |