1POA
INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2
Experimental procedure
| Spacegroup name | I 4 |
| Unit cell lengths | 93.000, 93.000, 22.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.300 * - 1.500 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.400 |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Rmerge | 0.050 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | drop | 1,4-butanediol | 20 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | 1,4-butanediol | 50 (%) | |
| 7 | 1 | reservoir | methyl alcohol | 10 (%) |
