1PN0

Phenol hydroxylase from Trichosporon cutaneum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2001-06-17
Detector	MARRESEARCH
Wavelength(s)	0.8452
Spacegroup name	P 1 21 1
Unit cell lengths	99.999, 150.948, 114.962
Unit cell angles	90.00, 114.63, 90.00

Refinement procedure

Resolution	20.000 * - 1.700
R-factor	0.15698
Rwork	0.157
R-free	0.17990 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	PDB ID ENTRY 1FOH
RMSD bond length	0.012
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.680	1.680
Rmerge	0.033 *	0.256 *
Number of reflections	297900	3440 *
<I/σ(I)>	23	2.2
Completeness [%]	83.9	38.7
Redundancy	2.1	0.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *	Enroth, C., (1994) J.Mol.Biol., 238, 128. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	drop	phenol	20 (mM)
3	1	reservoir	Tris-HCl	120 (mM)	or 0.21M KCl and 16% PEG4000
4	1	reservoir	ammonium acetate	0.52 (M)