1PN0
Phenol hydroxylase from Trichosporon cutaneum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8452 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.999, 150.948, 114.962 |
| Unit cell angles | 90.00, 114.63, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.15698 |
| Rwork | 0.157 |
| R-free | 0.17990 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB ID ENTRY 1FOH |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.033 * | 0.256 * |
| Number of reflections | 297900 | 3440 * |
| <I/σ(I)> | 23 | 2.2 |
| Completeness [%] | 83.9 | 38.7 |
| Redundancy | 2.1 | 0.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 4 * | Enroth, C., (1994) J.Mol.Biol., 238, 128. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 20 (mg/ml) | |
| 2 | 1 | drop | phenol | 20 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 120 (mM) | or 0.21M KCl and 16% PEG4000 |
| 4 | 1 | reservoir | ammonium acetate | 0.52 (M) |
