1PMA
PROTEASOME FROM THERMOPLASMA ACIDOPHILUM
Experimental procedure
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Detector technology | IMAGE PLATE |
Collection date | 1994-09 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 311.900, 209.700, 117.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.400 |
R-factor | 0.221 |
Rwork | 0.221 |
RMSD bond length | 0.010 |
RMSD bond angle | 21.916 * |
Data reduction software | MOSFLM (V. 5.23) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.200 |
Rmerge | 0.096 |
Number of reflections | 105440 |
Completeness [%] | 83.0 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | proteasomes | 7 (mg/ml) | |
2 | 1 | 1 | MPS | 5 (mM) | |
3 | 1 | 1 | EDTA | 1 (mM) | |
4 | 1 | 1 | 1 (mM) | ||
5 | 1 | 1 | PEG1000 | 10 (%) | |
6 | 1 | 1 | potassium phosphate | 0.1 (M) | |
7 | 1 | 2 | PEG1000 | 15 (%) | |
8 | 1 | 2 | potassium phosphate | 0.1 (M) |