1PM5
Crystal structure of wild type Lactococcus lactis Fpg complexed to a tetrahydrofuran containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.784, 91.784, 141.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| Rwork | 0.196 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnj |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (VERSION 1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 43717 | |
| <I/σ(I)> | 10.2 | 4 |
| Completeness [%] | 97.5 | 97.5 |
| Redundancy | 6.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | citrate, hepes, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | citrate | ||
| 2 | 1 | 1 | hepes | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | water | ||
| 5 | 1 | 2 | citrate | ||
| 6 | 1 | 2 | glycerol |
