1PJP
THE 2.2 A CRYSTAL STRUCTURE OF HUMAN CHYMASE IN COMPLEX WITH SUCCINYL-ALA-ALA-PRO-PHE-CHLOROMETHYLKETONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 301 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 74.370, 74.370, 50.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rp2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.810 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.254 |
| Total number of observations | 138168 * | |
| Number of reflections | 14122 | |
| Completeness [%] | 96.4 | 98.8 |
| Redundancy | 9.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 22 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MOPS | 10 (mM) | pH6.8 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | protein | 7.6 (mg/ml) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG10000 | 20 (%(v/v)) |
