1PJ6
Crystal structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with folic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 70.877, 222.506, 119.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.650 |
| R-factor | 0.15752 |
| Rwork | 0.156 |
| R-free | 0.19300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pj5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.634 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.067 | 0.346 * |
| Number of reflections | 106504 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 294 | 15% PEG 2000MME, 0.2 MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG5000 MME | 15 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | pH7.5 | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
