1PJ5
Crystal structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 71.381, 226.680, 120.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.600* |
| R-factor | 0.1619 |
| Rwork | 0.160 |
| R-free | 0.19800 * |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.096 | 0.521 * |
| Number of reflections | 114767 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 97.2 | 96.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 15 % PEG 2000MME, 0.2 M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG5000 MME | 15 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | pH7.5 | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
