1PFO
PERFRINGOLYSIN O
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | FILM |
Collection date | 1996-04 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.987, 178.292, 174.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.26800 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 27.950 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.058 | 0.410 |
Number of reflections | 37231 | |
<I/σ(I)> | 19.7 | 3.1 |
Completeness [%] | 86.0 | 87.1 |
Redundancy | 3.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.7 * | 22 * | Feil, S.C., (1996) FEBS Lett., 397, 290. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | reservoir | PEG20000 | 6 (%(w/v)) | |
3 | 1 | reservoir | bicine | 100 (mM) | |
4 | 1 | reservoir | dioxane | 2 (%(v/v)) |