1PEX

COLLAGENASE-3 (MMP-13) C-TERMINAL HEMOPEXIN-LIKE DOMAIN

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	52.940, 52.940, 144.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.195
Rwork	0.195
R-free	0.31900 *
RMSD bond length	0.017
RMSD bond angle	2.235
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.400
High resolution limit [Å]	2.700	2.700 *
Rmerge	0.063
Total number of observations	24238 *
Number of reflections	6858
Completeness [%]	95.7	94.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		5 (mM)
4	1	drop		300 (mM)
5	1	drop	ammonium sulfate	1 (M)
6	1	drop	sodium borate	0.1 (M)
7	1	reservoir	ammonium sulphate	1.2 (M)