1PDQ
Polycomb chromodomain complexed with the histone H3 tail containing trimethyllysine 27.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 32.120, 75.810, 80.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.760 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25230 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1KNA |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 * |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.033 * | 0.212 * |
| Number of reflections | 9494 | |
| <I/σ(I)> | 32.6 | 2.95 |
| Completeness [%] | 93.7 | 74.2 |
| Redundancy | 4.1 | 1.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 10 or 4 * | 0.1 M Tris-HCl, 0.2 M Lithium sulfate, 30 % PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | protein | 5 (mg/ml) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | PEG4000 | 30 (%) |
