1PB0
YCDX PROTEIN IN AUTOINHIBITED STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.130, 77.330, 82.280 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.950 |
| Rwork | 0.164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m65 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.081 | 0.212 |
| Number of reflections | 59787 | |
| <I/σ(I)> | 16.2 | 3.3 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris, 60% AS, 3% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
