1P8J
CRYSTAL STRUCTURE OF THE PROPROTEIN CONVERTASE FURIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.305, 135.392, 137.806 |
| Unit cell angles | 103.56, 98.98, 107.09 |
Refinement procedure
| Resolution | 18.830 * - 2.600 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.830 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 * | 0.347 * |
| Total number of observations | 382735 * | |
| Number of reflections | 182735 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 97.7 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 293 | 1.0M (NH4)2SO4, 0.4M Li2SO4, 0.1M Na3Citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | reservoir | 0.4 (M) | ||
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH6.0 |
