1P5J

Crystal Structure Analysis of Human Serine Dehydratase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-12-25
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	157.436, 157.436, 59.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.189
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tdj
RMSD bond length	0.012
RMSD bond angle	1.623
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.099
Number of reflections	12345
Completeness [%]	93.6	96.3
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	(NH4)2SO4, Tris, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K