1P4L

Crystal structure of NK receptor Ly49C mutant with its MHC class I ligand H-2Kb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.916
Spacegroup name	P 43 21 2
Unit cell lengths	152.010, 152.010, 64.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.900
Rwork	0.269
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2VAB and 1QO3
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.600	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.062 *	0.325
Number of reflections	16931	840 *
Completeness [%]	97.5	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Protein 8mg/ml, 0.1 M HEPES-Na pH 7.5, 2% v/v PEG400, 2.0 M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-10 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	PEG400	2 (%(v/v))
4	1	reservoir	HEPES	0.1 (M)	pH7.5