1P4K
CRYSTAL STRUCTURE OF THE GLYCOSYLASPARAGINASE PRECURSOR D151N MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.176, 52.717, 61.924 |
| Unit cell angles | 80.85, 90.21, 105.08 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.182 |
| R-free | 0.21980 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.020 * | 0.040 * |
| Total number of observations | 80492 * | |
| Number of reflections | 43700 * | |
| Completeness [%] | 95.1 * | 95.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse matrix sampling method * | 7.5 | 283 * | Cui, T., (1999) Acta Crystallogr., D55, 1961. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 10 (mM) | pH7.4 |
| 2 | 1 | 1 | 50 (mM) | ||
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 1 | glycine | 20 (mM) | |
| 5 | 1 | 1 | protein | 5 (mg/ml) | |
| 6 | 1 | 2 | PEG8000 | 8 (%) | |
| 7 | 1 | 2 | PEG4000 | 30 (%) | |
| 8 | 1 | 2 | lithium sulfate | 0.2 (M) | pH8.5 |
| 9 | 1 | 2 | Tris | 0.1 (M) |
