1P4H
Crystal structure of glycogen phosphorylase b in complex with C-(1-acetamido-alpha-D-glucopyranosyl) formamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8015 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.980, 127.980, 115.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 2.060 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.20500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1gfz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 59423 | |
<I/σ(I)> | 17.1 | 2.9 |
Completeness [%] | 99.4 | 92.8 |
Redundancy | 6.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 289K |