1P3Q
Mechanism of Ubiquitin Recognition by the CUE Domain of VPS9
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2002-10-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979174, 0.979311, 0.95645 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.607, 45.887, 57.802 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.700 |
Rwork | 0.260 |
R-free | 0.27700 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.367 |
Number of reflections | 28955 | 2626 * |
<I/σ(I)> | 2.3 | |
Completeness [%] | 98.4 | 89.6 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 293 | PEG 3350, magnesium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | Tris | 20 (mM) | pH7.7 |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.9-2.1 (M) | |
6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2-8.8 |