1P36
T4 LYOSZYME CORE REPACKING MUTANT I100V/TA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.843, 59.843, 95.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 1.450 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.110 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.238 |
| Number of reflections | 35041 | |
| <I/σ(I)> | 8.1 | 3.1 |
| Completeness [%] | 97.9 | 86 * |
| Redundancy | 7.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 | 277 | Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | phosphate | 2.0 (M) | |
| 4 | 1 | reservoir | beta-mercaptoethanol |
