1P2D
Crystal Structure of Glycogen Phosphorylase B in complex with Beta Cyclodextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8068 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.873, 128.873, 116.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.940 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gfz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.960 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.076 * | 0.499 * |
| Total number of observations | 714419 * | |
| Number of reflections | 68504 | |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 93.4 | 85 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 289 | Oikonomakos, N.G., (2000) J.Biol.Chem., 275, 34566. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 25 (mg/ml) | |
| 2 | 1 | 1 | flavopiridol | 1 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 4 | 1 | 1 | Bes | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%) | |
| 7 | 1 | 1 | spermine | 1 (mM) | or 10 mM magnesium acetate |
| 8 | 1 | 1 | glucose | 50 (mM) |
