1P1Z
X-RAY CRYSTAL STRUCTURE OF THE LECTIN-LIKE NATURAL KILLER CELL RECEPTOR LY-49C BOUND TO ITS MHC CLASS I LIGAND H-2Kb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 149.047, 149.047, 64.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.260 |
| Rwork | 0.263 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.520 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.260 | 3.260 |
| Rmerge | 0.096 | 0.264 * |
| Number of reflections | 12168 * | 596 * |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 99.5 | 99.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 4 * | 10% PEG 20000, 2% DIOXANE, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG20000 | 10 (%(w/v)) | |
| 3 | 1 | reservoir | dioxane | 2 (%(v/v)) | |
| 4 | 1 | reservoir | bicine | 0.1 (M) | pH9.0 |
