1P1Y
Crystal structure of a continuous three-dimensional DNA lattice from d(GGACAGATGGGAG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92002 |
| Spacegroup name | P 64 |
| Unit cell lengths | 40.630, 40.630, 55.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.140 - 2.100 |
| R-factor | 0.20485 |
| Rwork | 0.203 |
| R-free | 0.23256 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 1.800 | 2.100 |
| Rmerge | 0.071 | 0.220 |
| Number of reflections | 3066 | |
| <I/σ(I)> | 20.7 | 13.76 |
| Completeness [%] | 99.0 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.5 | magnesium formate, mpd, lithium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium formate | ||
| 2 | 1 | 1 | mpd | ||
| 3 | 1 | 1 | LiCl | ||
| 4 | 1 | 2 | mpd | ||
| 5 | 1 | 2 | LiCl |
