1P15

Crystal structure of the D2 domain of RPTPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	105
Wavelength(s)	1.08
Spacegroup name	P 31
Unit cell lengths	59.101, 59.101, 152.657
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.180 - 2.000
R-factor	0.203
Rwork	0.203
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.100	2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.041 *	0.402 *
Total number of observations	442014 *
Number of reflections	40834
Completeness [%]	96.0	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	4 *	PEG 8000, sodium succinate, lithium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15 (%(w/v))
2	1	reservoir	sodium succinate	0.1 (M)	pH5.5
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir		0.25 (M)
5	1	drop	protein	12 (mg/ml)