1OYR
Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.790, 146.490, 152.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 3.100 |
Rwork | 0.278 |
R-free | 0.28890 * |
Structure solution method | MIR |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | |
High resolution limit [Å] | 3.100 | 3.100 * |
Rmerge | 0.116 * | 0.372 * |
Number of reflections | 40776 | 2021 * |
Completeness [%] | 99.1 * | 99.8 * |
Redundancy | 5.2 * | 4.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 37.5 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
3 | 1 | reservoir | 20 (mM) |