1OYP
Crystal Structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.310, 163.560, 166.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.750* |
Rwork | 0.285 |
R-free | 0.30600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | |
High resolution limit [Å] | 2.750 * | 2.750 * |
Rmerge | 0.097 * | 0.332 * |
Number of reflections | 36688 * | 1753 * |
Completeness [%] | 99.2 * | 96.6 * |
Redundancy | 5.9 * | 4.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12.5 (mg/ml) | |
2 | 1 | reservoir | 1.0 (M) | ||
3 | 1 | reservoir | 3.0 (M) |