1OW0
Crystal structure of human FcaRI bound to IgA1-Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 3 |
| Unit cell lengths | 142.987, 142.987, 67.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 3.100 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1DN2 1ovz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.089 | 0.310 |
| Total number of observations | 213861 * | |
| Number of reflections | 27807 * | |
| <I/σ(I)> | 12 | 2.4 |
| Completeness [%] | 95.8 | 71.6 |
| Redundancy | 7.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | magnesium sulfate heptahydrate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 3 | 1 | drop | magnesium sulfate | 1.7-1.85 (M) | |
| 4 | 1 | drop | MES | 0.1 (M) | pH6.5-6.7 |
