1OW0
Crystal structure of human FcaRI bound to IgA1-Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95 |
Spacegroup name | P 3 |
Unit cell lengths | 142.987, 142.987, 67.655 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 * - 3.100 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1DN2 1ovz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.520 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.089 | 0.310 |
Total number of observations | 213861 * | |
Number of reflections | 27807 * | |
<I/σ(I)> | 12 | 2.4 |
Completeness [%] | 95.8 | 71.6 |
Redundancy | 7.7 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | magnesium sulfate heptahydrate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
3 | 1 | drop | magnesium sulfate | 1.7-1.85 (M) | |
4 | 1 | drop | MES | 0.1 (M) | pH6.5-6.7 |