1ORV

Crystal Structure of Porcine Dipeptidyl Peptidase IV (CD26)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-12
Detector	MARRESEARCH
Wavelength(s)	1.05030
Spacegroup name	P 1
Unit cell lengths	62.000, 118.180, 133.590
Unit cell angles	112.76, 94.93, 91.14

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.217
Rwork	0.217
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MAIN
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.052 *	0.352 *
Total number of observations	617050 *
Number of reflections	322283 *
Completeness [%]	96.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG 2000, 0.1 M ammonium sulfate, 0.1 M Tris/HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG 2000, 0.1 M ammonium sulfate, 0.1 M Tris/HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG2000	20-22 (%)
3	1	reservoir	ammonium sulfate	0.1 (M)
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.0