1ORS
X-ray structure of the KvAP potassium channel voltage sensor in complex with an Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.710, 61.600, 46.060 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 1BAF |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 56778 | |
| <I/σ(I)> | 15.5 | 5.8 |
| Completeness [%] | 96.7 | 92.8 |
| Redundancy | 2.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG4000, Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
