1OQB
The Crystal Structure of the one-iron form of the di-iron center in Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.908, 145.791, 192.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.22477 |
| Rwork | 0.224 |
| R-free | 0.24800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.058 * | 0.252 * |
| Number of reflections | 45472 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 79.3 | 32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 293 | 8-12% Peg8000, 50mM Potassium Dihydrogen Phosphate and 20% Glycerol as cryoprotectant, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium azide | 70 (mM) | |
| 2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
| 3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
| 5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
| 6 | 1 | reservoir | PEG4000 | 12-15 (%) |
