1OQB
The Crystal Structure of the one-iron form of the di-iron center in Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-29 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8423 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.908, 145.791, 192.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.22477 |
Rwork | 0.224 |
R-free | 0.24800 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.240 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.058 * | 0.252 * |
Number of reflections | 45472 | |
<I/σ(I)> | 12.1 | |
Completeness [%] | 79.3 | 32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 293 | 8-12% Peg8000, 50mM Potassium Dihydrogen Phosphate and 20% Glycerol as cryoprotectant, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium azide | 70 (mM) | |
2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
6 | 1 | reservoir | PEG4000 | 12-15 (%) |