1OOI
Crystal structure of LUSH from Drosophila melanogaster at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 55.370, 55.370, 66.946 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.040 |
| R-factor | 0.20083 |
| Rwork | 0.195 |
| R-free | 0.24700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oof |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.172 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.054 | 0.247 |
| Total number of observations | 112114 * | |
| Number of reflections | 7504 | |
| <I/σ(I)> | 57.9 | 10.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.9 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 291 | PEG 4000, MOPS, n-butanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 100 (mM) | pH8.0 |
| 2 | 1 | drop | n-butanol | 0.3 (%(v/v)) | |
| 3 | 1 | reservoir | MOPS | 100 (mM) | pH6.5 |
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 5 | 1 | reservoir | n-butanol | 0.3 (%(v/v)) |
