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Crystal structure of the Drosophila Mago nashi-Y14 complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.027, 52.387, 52.862 |
Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.26100 * |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 23.600 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.051 * | 0.346 * |
Total number of observations | 155205 * | |
Number of reflections | 23222 * | |
Completeness [%] | 97.5 | 81.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 290 | MPD, sodium acetate, Strontium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
1 | Vapor diffusion * | 8 * | 290 | MPD, sodium acetate, Strontium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-30 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | pH8.0 |
3 | 1 | drop | glycerol | 5 (%) | |
4 | 1 | drop | 500 (mM) | ||
5 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.8 |
6 | 1 | reservoir | MPD | 30 (%) | |
7 | 1 | reservoir | 10 (mM) |