1OO0

Crystal structure of the Drosophila Mago nashi-Y14 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9500
Spacegroup name	C 1 2 1
Unit cell lengths	105.027, 52.387, 52.862
Unit cell angles	90.00, 104.90, 90.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.215
Rwork	0.215
R-free	0.26100 *
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	23.600 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850	1.850
Rmerge	0.051 *	0.346 *
Total number of observations	155205 *
Number of reflections	23222 *
Completeness [%]	97.5	81.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	290	MPD, sodium acetate, Strontium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	Vapor diffusion *	8 *	290	MPD, sodium acetate, Strontium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH8.0
3	1	drop	glycerol	5 (%)
4	1	drop		500 (mM)
5	1	reservoir	sodium acetate	100 (mM)	pH5.8
6	1	reservoir	MPD	30 (%)
7	1	reservoir		10 (mM)