1OMD
STRUCTURE OF ONCOMODULIN REFINED AT 1.85 ANGSTROMS RESOLUTION. AN EXAMPLE OF EXTENSIVE MOLECULAR AGGREGATION VIA CA2+
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.590, 64.280, 33.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.850 |
| R-factor | 0.166 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 0.044 * |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.850 * |
| Rmerge | 3.000 * |
| Number of reflections | 5965 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.2 * | 20 * | took Przybylska et al., from original paper * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.4 (%) | |
| 2 | 1 | drop | cacodylate | 5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | PEG6000 | 11 (%) | |
| 6 | 1 | reservoir | PEG6000 | 33 (%(w/v)) | |
| 7 | 1 | reservoir | cacodylate | 10 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 1 (mM) |
