1OLM
Supernatant Protein Factor in Complex with RRR-alpha-Tocopherylquinone: A Link between Oxidized Vitamin E and Cholesterol Biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.856, 84.621, 87.452 |
| Unit cell angles | 116.20, 102.64, 100.24 |
Refinement procedure
| Resolution | 19.650 - 1.950 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.21100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.059 | 0.340 |
| Number of reflections | 103050 | |
| <I/σ(I)> | 14.9 | 3.2 |
| Completeness [%] | 100.0 | 71.6 |
| Redundancy | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | pH 8.30 |
