1OKC
structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Collection date | 2001-06-15 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.437, 83.463, 49.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 2.200 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26800 * |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 17.900 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.517 * |
Number of reflections | 18544 | |
<I/σ(I)> | 7.6 | 1.4 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 20 * | 30% JEFFAMINE600, 5MM NA CITRATE, 100MM TRIS PH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Jeffamine-M600 | 28-32 (%) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | drop | 5 (mM) | or 100mM |